Journal: Cellular and Molecular Life Sciences

Article Title: Protease-independent control of parthanatos by HtrA2/Omi

doi: 10.1007/s00018-023-04904-7

Figure Lengend Snippet: Mitochondrial proteins with statistically significant differences in their abundance in untreated and MNNG-treated WT vs. HtrA2/Omi KO MEF [ANOVA of log 2 -transformed scaled abundances using 5% FDR and post-hoc Tukey’s HSD test (5%)]

Article Snippet: Cells were incubated overnight with primary antibody (anti-HtrA2/Omi rabbit polyclonal IgG (15775-1-AP, Proteintech) for parental or HtrA2/Omi-deficient cells, anti-HtrA2/Omi rabbit polyclonal IgG (AF1458, R&D Systems) for HtrA2/Omi-reconstituted cells, or anti-FLAG M2 mouse monoclonal IgG (F1804, Sigma–Aldrich) for PARL-FLAG-reconstituted cells, all 1:200 in TBS with 1% (v/v) BSA), washed four times in TBS (8 min each), incubated for 2 h at room temperature with secondary antibody [goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (A-11008, ThermoFisher Scientific) or goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (A-11001, ThermoFisher Scientific), both 1:1000 in TBS with 1% (v/v) BSA] with addition of Hoechst 33342 dye (1:1000, ThermoFisher Scientific) to stain the nuclei, washed four times in TBS (8 min each), once in Milli-Q water (2 min) and then analyzed using a Zeiss LSM 510 confocal laser scanning microscope (Zeiss, Oberkochen, Germany).

Techniques: Transformation Assay

Journal: Cellular and Molecular Life Sciences

Article Title: Protease-independent control of parthanatos by HtrA2/Omi

doi: 10.1007/s00018-023-04904-7

Figure Lengend Snippet: Mitochondrial proteins with statistically significant differences in their abundance in untreated and MNNG-treated WT vs. HtrA2/Omi KO MEF [ANOVA of scaled abundances using 5% FDR and post-hoc Tukey’s HSD test (5%), and additional pairwise comparison of groups by Student’s t test]

Article Snippet: Cells were incubated overnight with primary antibody (anti-HtrA2/Omi rabbit polyclonal IgG (15775-1-AP, Proteintech) for parental or HtrA2/Omi-deficient cells, anti-HtrA2/Omi rabbit polyclonal IgG (AF1458, R&D Systems) for HtrA2/Omi-reconstituted cells, or anti-FLAG M2 mouse monoclonal IgG (F1804, Sigma–Aldrich) for PARL-FLAG-reconstituted cells, all 1:200 in TBS with 1% (v/v) BSA), washed four times in TBS (8 min each), incubated for 2 h at room temperature with secondary antibody [goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (A-11008, ThermoFisher Scientific) or goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (A-11001, ThermoFisher Scientific), both 1:1000 in TBS with 1% (v/v) BSA] with addition of Hoechst 33342 dye (1:1000, ThermoFisher Scientific) to stain the nuclei, washed four times in TBS (8 min each), once in Milli-Q water (2 min) and then analyzed using a Zeiss LSM 510 confocal laser scanning microscope (Zeiss, Oberkochen, Germany).

Techniques: Comparison

Journal: Cellular and Molecular Life Sciences

Article Title: Protease-independent control of parthanatos by HtrA2/Omi

doi: 10.1007/s00018-023-04904-7

Figure Lengend Snippet: Mitochondrial peptides with statistically significant differences in their abundance in untreated and MNNG-treated WT vs. HtrA2/Omi KO MEF [ANOVA of scaled abundances using 5% FDR and post-hoc Tukey’s HSD test (5%), and additional pairwise comparison of groups by Student’s t test]

Article Snippet: Cells were incubated overnight with primary antibody (anti-HtrA2/Omi rabbit polyclonal IgG (15775-1-AP, Proteintech) for parental or HtrA2/Omi-deficient cells, anti-HtrA2/Omi rabbit polyclonal IgG (AF1458, R&D Systems) for HtrA2/Omi-reconstituted cells, or anti-FLAG M2 mouse monoclonal IgG (F1804, Sigma–Aldrich) for PARL-FLAG-reconstituted cells, all 1:200 in TBS with 1% (v/v) BSA), washed four times in TBS (8 min each), incubated for 2 h at room temperature with secondary antibody [goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (A-11008, ThermoFisher Scientific) or goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (A-11001, ThermoFisher Scientific), both 1:1000 in TBS with 1% (v/v) BSA] with addition of Hoechst 33342 dye (1:1000, ThermoFisher Scientific) to stain the nuclei, washed four times in TBS (8 min each), once in Milli-Q water (2 min) and then analyzed using a Zeiss LSM 510 confocal laser scanning microscope (Zeiss, Oberkochen, Germany).

Techniques: Comparison